ABSTRACT
Human fascioliasis is another important health challenge in Egypt. lip till now and due to many factors. its diagnosis is a problematic issue. In many eases, a considerable damage of the hepatic tissue often occurs before a proper diagnosis could he done. This in turn necessitates finding a reliable. easily applicable and locally affordable diagnostic test to overcome the diagnostic difficulties that handicap prevention and control efforts. The detection of E/S antigens in stool specimens [coproantigens] and in sera of infected humans using a MAb-based sandwich ELISA system. Stool and serum samples were collected from 35 fascioliasis patients having clinical and parasitological evidences of infection, 20 patients harboring other parasites [Schistosoma mansoni and haematobium Wuchereria bancuofti and hydatid cysts] and 25 healthy subjects. A pair of monoelonal antibodies [MAbs: 9F/10B and 5F/6H], raised against both FascioIa gigantica excretory-Secretor [E/S] products and crude antigens respectively, were employed in sandwich ELISA. The lower detection limit of E/S coproantigen assay corresponded to 15 ng/ml, while that of E/S antigen assay in serum corresponded to 50ng/ml. The anti-F gigantica MAb-based sandwich ELISA for antigen detection in collected sera showed 77% sensitivity and 100% specificity with 87% diagnostic accuracy. Coproantigen detection in stool samples showed 94% sensitivity and 100% specificity with 97% diagnostic accuracy. A positive correlation was detected between antigen level in stool samples and its level in corresponding serum samples. This study showed that the use of anti-F gigantica MAb-based sandwich ELISA was more ssensitive for antigen detection in stool samples of fascioliasms patients. Than its detection in their corresponding serum samples, providing a simple, reliable, non-invasive diagnostic method for active human infection
ABSTRACT
Artificial insemination by husband's semen [AIH] was done for 36 females with variable causes of infertility [male factor, cervical factor and unexplained infertility] with at least one patient healthy tube. 101 trials were done during the period of the study. Transuterine intratubal insemination was done using intrauterine foley catheter. Success of the procedure was first tested during diagnostic laparoscopy for infertility and vaginal ultrasonography [US]. Assessment of true trials was done by vaginal US alone. In all the trials, the treated semen was successfully placed into the patent fallopian tubes. This easy, rapid and convenient technique should be the first line of choice when AIH is indicated
Subject(s)
FemaleABSTRACT
Artificial insemination by husband's semen [AIH] was done for 36 females with variable causes of infertility [male factor, cervical factor and unexplained infertility] with at least one patient healthy tube. 101 trials were done during the period of the study. Transuterine intratubal insemination was done using intrauterine foley catheter. Success of the procedure was first tested during diagnostic laparoscopy for infertility and vaginal ultrasonography [US]. Assessment of true trials was done by vaginal US alone. In all the trials, the treated semen was successfully placed into the patent fallopian tubes. This easy, rapid and convenient technique should be the first line of choice when AIH is indicated